Journal: bioRxiv

Article Title: OMA1 mediates local and global stress responses against protein misfolding in CHCHD10 mitochondrial myopathy

doi: 10.1101/2021.12.21.473493

Figure Lengend Snippet: (A) Representative immunoblot of eIF2α and p-eIF2α from heart and muscle lysates of 37–44-week-old C10 WT , C10 G58R , and C10 S59L mice. *Nonspecific band. (B) Quantification of (A), (n = 4 mice per genotype). (C) Microarray data of hearts from 14-week-old C10 G58R vs C10 WT and 20-week-old C10 S59L vs C10 WT mice, all on the OMA1 +/- background. Each column represents a mouse, and q-values represent genome-wide significance. (D) Timeline of the ASO experiment. (E) Immunoblots of OPA1, OMA1, eIF2α, and p-eIF2α of C10 G58R mouse hearts on the OMA1 +/- background treated with either a nontargeting (CTRL) or OMA1 ASO. Loading controls for eIF2α and p-eIF2α in Figure S6F. *Nonspecific band. (F) Quantification of (E), (n = 3 mice CTRL ASO and n = 4 mice OMA1 ASO). (G) Microarray data of 33-week-old C10 G58R mouse hearts on the OMA1 +/- background treated with either a nontargeting (CTRL) or OMA1 ASO. Each column represents a mouse, and q-values represent genome-wide significance. (H) GSEA of reactome pathways of the listed comparisons. Pathways shown are all the pathways with a q-value < 0.025 in the C10 G58R OMA1 ASO vs CTRL ASO comparison. Error bars represent SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. See also Figure S6 and Supplemental Files 2 and 3.

Article Snippet: RNA expression was measured using the Clariom_S_Mouse microarray (Affymetrix).The Transcriptome Analysis Console software (Affymetrix, version 4.0.1) was used to analyze the data with the default settings.

Techniques: Western Blot, Microarray, Genome Wide